mouse cmt 93 ccl 223 colon epithelial cell line  (ATCC)

Journal: PLoS ONE

Article Title: Regulation of Serum Amyloid A3 (SAA3) in Mouse Colonic Epithelium and Adipose Tissue by the Intestinal Microbiota

doi: 10.1371/journal.pone.0005842

Figure Lengend Snippet: (A) qRT-PCR analysis of SAA3 mRNA expression levels along the length of the gut in germ-free (GF) and conventionally raised (CONV-R) mice using pooled cDNA samples (n = 5 mice per group). Duo , duodenum; Jej , jejunum; Ile , ileum; Col , proximal colon. Data are means±SD. (B) Colonic SAA3 mRNA expression assayed in individual mice (n = 8−10 mice per group). Data are means±SEM. * P <0.05; Student's t -test. (C) Representative immunohistochemical staining of CONV-R mouse colonic sections shows bis -benzimide-positive nuclei ( blue ) and SAA3 ( green ) expression in surface epithelial cells. (D) Primary antibody omitted. Scale bars = 30 µm.

Article Snippet: Both the mouse colonic epithelial cell line (CMT-93) and the macrophage cell line (RAW 264.7) were purchased from ATCC (Manassas, VA) and maintained in Dulbecco's Modified Eagle Medium (PAA Laboratories) containing 100 U/mL penicillin, 100 μg/ml streptomycin, 2 mM L -glutamine, 2 mM sodium pyruvate and 10% fetal calf serum.

Techniques: Quantitative RT-PCR, Expressing, Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: Regulation of Serum Amyloid A3 (SAA3) in Mouse Colonic Epithelium and Adipose Tissue by the Intestinal Microbiota

doi: 10.1371/journal.pone.0005842

Figure Lengend Snippet: SAA3 and TNF-α activation in the colon may occur upon ( left panel ) direct stimulation of epithelial cells by LPS or translocation of LPS across compromised tight junctions. ( upper right panel ) Colonic epithelial cells (EC) express TLR4 and respond to LPS stimulation by importing NF-κB to the nucleus where it can bind to DNA regulatory elements and promote expression of SAA3. LPS also stimulates the expression of TNF-α, another cytokine influencing local and systemic inflammation. TNF-α may further stimulate SAA3 expression in the intestinal epithelium. ( lower right panel ) Macrophages (MΦ) also express TLR4 and respond to LPS stimulation by promoting transcription of TNF-α mRNAs.

Article Snippet: Both the mouse colonic epithelial cell line (CMT-93) and the macrophage cell line (RAW 264.7) were purchased from ATCC (Manassas, VA) and maintained in Dulbecco's Modified Eagle Medium (PAA Laboratories) containing 100 U/mL penicillin, 100 μg/ml streptomycin, 2 mM L -glutamine, 2 mM sodium pyruvate and 10% fetal calf serum.

Techniques: Activation Assay, Translocation Assay, Expressing